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简短的糖蛋白识别蛋白5调节了印度大鱼,Cirrhinusmrigalagala中的细菌的免疫反应

Zahoor Mushtaq¹, Pani Prasad Kurcheti¹, K Jeena¹

¹ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India.

Developmental and comparative immunology

|December 1, 2023

概括

在鱼中发现了一种新的糖蛋白识别蛋白5 (PGRP5) 基因mpgrp5. 这种基因在鱼中起着至关重要的作用.

科学领域:

水生免疫学水生免疫学
鱼类分子生物学鱼类分子生物学
这是天生的免疫力.

背景情况:

甘氨酸识别蛋白 (PGRP) 是天生的免疫系统的关键组成部分,能够识别细菌甘氨酸 (PGN).
了解鱼类中的PGRP功能对于水产养殖健康和疾病管理至关重要.

研究的目的:

在鱼 (Cirrhinus mrigala) 中识别和描述一种新的PGRP5基因 (mpgrp5).
研究mpgrp5在早期发育过程中的表达模式以及对细菌挑战的反应.

主要方法:

cDNA测序和生物信息学分析以表征mpgrp5基因和蛋白质.
定量PCR (qPCR) 用于分析本体遗传和组织特异性表达.
基因表达分析的后细菌挑战.

主要成果:

确定了1255bp的全长mpgrp5cDNA,它编码了一个具有保留PGRP/阿米达酶域的248氨基酸蛋白.
遗传学分析显示mpgrp5与其他鱼类的PGRP密切相关.
mpgrp5在化7天后表达最高,在大多数组织 (肠道最高) 中具有构成性表达,并在肝脏,脏,,皮肤和脏的细菌挑战后显著上调.

结论:

鉴定到的mpgrp5基因具有功能性PGRP的特征.
mpgrp5很可能从早期发育阶段就参与mrigal鱼的抗菌免疫反应.
这些发现有助于理解鱼类的先天免疫力和潜在的疾病抵抗机制.

关键词:

这种植物是Aeromonas hydrophila.阿米达斯的活性活动.这种植物名为Cirrhinus mrigala.天生的免疫反应.我们的PGRP是PGRP.

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