在Saccharomyces cerevisiae中完整的还原性甘氨酸途径的工程和演化,用于格式和CO2的同化
Viswanada R Bysani1, Ayesha S Alam1, Arren Bar-Even1
1Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.
Metabolic engineering
|December 1, 2023
概括
工程酵母Saccharomyces cerevisiae利用生物质的形式和二氧化碳 (CO2),使循环碳经济成为可能. 适应性实验室进化显著增强了生长,证明了可用于二氧化碳同化的功能性降低性甘氨酸通路 (RGP).
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 捕获的二氧化碳 (CO2) 和酸盐等C1原料对于循环碳经济至关重要.
- 工程酵母,Saccharomyces cerevisiae,用于形式和二氧化碳同化可以推进形式介导的循环生物经济.
研究的目的:
- 通过减少性甘氨酸通路 (RGP) 来设计酵母以形成酸盐和二氧化碳同化.
- 通过适应性实验室进化 (ALE) 加强RGP活动,并使这些原料的增长成为可能.
主要方法:
- 在Saccharomyces cerevisiae中RGP血清合成模块的模块化实现.
- 增长合选择和适应性实验室进化 (ALE) 用于路径优化.
- 13C标记实验用于追踪碳流并确认通路活动.
主要成果:
- 从酵母中的甲酸盐和二氧化碳中建立了甘氨酸和氨酸的合成.
- 随着ALE的出现,RGP依赖的增长率提高了8倍.
- 证明了完全的RGP活动,碳从酸盐转移到酸盐,再加上二氧化碳同化.
- 鉴定并逆向工程GDH1突变增强格式同化.
结论:
- 降低性甘氨酸通路 (RGP) 可以在Saccharomyces cerevisiae中功能性地实现,以形成和二氧化碳同化.
- 适应性实验室进化 (ALE) 有效地提高了工程路径的性能.
- 工程酵母菌株显示了通过利用二氧化碳推进循环碳经济的潜力.
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