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一种新型的短型糖体识别蛋白,具有独特的多糖体识别特异性,在海,Apostichopus japonicus中发现

Jingwei Jiang¹, Shan Gao¹, Zelong Zhao¹

¹Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Liaoning Key Laboratory of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, PR China.

Fish & shellfish immunology

|December 1, 2023

概括

一种新的海免疫蛋白,AJPGRP-S3,可以识别广泛的病原体. 这种甘识别蛋白 (PGRP) 与各种免疫因子相互作用,突出其在海洋无脊椎动物防御信号传递中的作用.

科学领域:

海洋无脊椎动物免疫学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

模式识别受体 (PRR) 对于无脊椎动物的免疫力至关重要.
糖识别蛋白 (PGRP) 是关键的PRR,在无脊椎动物中具有多种异型.
了解像海黄瓜 (Apostichopus japonicus) 这样的生态重要物种中的PGRP至关重要.

研究的目的:

在海黄瓜Apostichopus japonicus中识别和描述一种新的短型PGRP,AJPGRP-S3.
研究 AjPGRP-S3.3 的基因表达模式和病原体识别能力.
为了探索 AjPGRP-S3 与其他免疫相关因素的蛋白质-蛋白质相互作用.

主要方法:

用快速放大cDNA末端的聚合酶连锁反应 (PCR-RACE) 进行全长cDNA测序.
在现场杂交,用于分析基因表达局部化.
重组蛋白表达和净化用于功能测定.
酵母二杂化用于识别蛋白质相互作用.

主要成果:

成功获得了AjPGRP-S3的全长cDNA.
AjPGRP-S3在胆红细胞和管脚中显示出高表达.
再组合的AjPGRP-S3表现出对格拉姆阴性和阳性细菌和真菌的广泛识别.
AjPGRP-S3与补体系统,细胞外基质,囊泡贩运和抗氧化剂系统蛋白质相互作用.

结论:

AjPGRP-S3在海黄的天生的免疫反应中起着重要作用.
这种PGRP参与病原体信号转导到下游免疫因子.
这些发现有助于理解海黄PGRP异型之间的功能多样性.

关键词:

免疫信号传导转导免疫信号传导异形酶是一种异形酶.莱克补充系统的补充系统类甘油识别蛋白质类甘油识别蛋白质海黄瓜Apostichopus japonicusus海黄瓜是日本海黄瓜的一种.

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