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在Aureobasidium pullulans中设计了还原性三碳酸途径,以增强聚-L-酸的生物合成

Zhen Qin¹, Jun Feng², You Li²

¹William G. Lowrie Department of Chemical & Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, Columbus, OH 43210, USA; Department of Food Science and Technology, The Ohio State University, 2015 Fyffe Road, Columbus, OH 43210, USA.

Bioresource technology

|December 1, 2023

概括

Aureobasidium pullulans 的基因工程增强了聚-L-酸 (PMA) 产量,通过促进减少性三碳酸 (rTCA) 途径中的关键酶. 这导致了显著提高产量和生产率,为工业应用铺平了道路.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

乌雷奥巴西普鲁兰斯天然产生聚L-酸 (PMA),一种具有工业潜力的生物聚合物.
目前A. pullulans的PMA生产表现出低的生产率和产量,阻碍了商业可行性.

研究的目的:

为工程师A. pullulansZX-10进行增强的PMA生产.
调查减少型三碳酸 (rTCA) 途径在PMA生物合成中的作用.

主要方法:

对A. pullulans ZX-10进行基因工程,以过度表达细胞质酸脱酶 (MDH),PYC和PMA合成酶 (PMS).
采用了一种高复制的酵母病性等离子体,其中含有来自Aspergillus nidulans的gpdA促进剂.
进行了摇瓶和料批发发酵,以评估PMA生产.

主要成果:

过度表达PMS,MDH和PYC在摇瓶培养中增加了19%-37%的PMA产量.
工程 A. pullulans 在料批发发酵中实现了 96.7 g/L PMA,生产率为 0.90 g/L·h,产量为 0.68 g/g 葡萄糖.
证明了rTCA途径在促进PMA生物合成中的关键作用.

结论:

在rTCA途径中增强关键酶的表达显著改善了A. pullulans.中的PMA产生.
该工程菌株显示出高产量和生产率,使其对工业规模的PMA制造具有前景.
对rTCA途径的代谢工程是增加生物聚合物生产的有效策略.

关键词:

乌雷奥巴西迪亚姆 (Aureobasidium Pullulans) 是一个有毒的植物.马拉酸脱酶的使用方法这就是PMA合成酶.酸盐碳氧化酶是什么?降低性TCA通路的方法

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