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多位点测序类型化揭示了Sporothrix brasiliensis的地理相关的物种内部变异性

Vanessa Brito de Souza Rabello¹, Marcus de Melo Teixeira², Wieland Meyer³

¹Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas (INI), Fundação Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil.

Fungal genetics and biology : FG & B

|December 1, 2023

概括

针对Sporothrix brasiliensis开发了一种新的多部位序列类型 (MLST) 方案,这种真菌会导致运动三症. 这个工具有助于追踪病原体.

科学领域:

医学真菌学医学真菌学
分子流行病学分子流行病学
遗传学遗传学是一个

背景情况:

脊髓炎是一种真菌感染,主要是由Sporothrix物种引起的.
葡萄 (Sporothrix brasiliensis) 是一种在南美洲,特别是巴西普遍存在的高度毒性物种,可以在动物和人类之间传播.
了解S. brasiliensis的遗传多样性对于流行病学监测和控制抗真菌耐药性至关重要.

研究的目的:

开发和验证一种新的多位子序列类型化 (MLST) 方案,用于Sporothrix brasiliensis.
分析来自不同地理来源的S. brasiliensis分离物体内的遗传变异.
建立一个强大的基因型定型方法,用于对胞三症的流行病学调查.

主要方法:

基于可用的S. brasiliensis基因组序列,使用Primer-BLAST设计了16个组成基因的原始基因.
测序了来自巴西和巴拉圭的91个人类,动物和环境S. brasiliensis分离物.
选择了具有高核酸多样性 (π) 的四个位点来建立MLST方案,标准化PCR条件.

主要成果:

新的MLST方案成功地将所有S. brasiliensis分离物分为七个不同的单独类型.
核酸多样性分析确定了四个高度可变的适合基因定型的基因位点.
一些已识别的单元类型与分离物的地理来源存在相关性,这表明了区域遗传结构.

结论:

开发的MLST计划显著增强了S. brasiliensis.基因定型的歧视权.
这种工具为巴西及其他地区的S. brasiliensis分子流行病学研究提供了宝贵的进步.
有助于更好地了解病原体的种群结构和传播,有助于公共卫生对抗运动三症的努力.

关键词:

流行病学流行病学遗传的变异性遗传的变异性分子类型的定型.球体三症是一种病.

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