作为替代氧化捐赠体的候选分子,具有更好的抗菌特性,可以对抗大肠杆菌和金黄色葡萄球菌
Jingjing Guo1, Xiaojing Tian1, Sihong Chen1
1College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Journal of applied microbiology
|December 1, 2023
概括
合成了四种新型氧化 (NO) 捐赠体,它们对大肠杆菌和金黄色杆菌具有显著的抗菌特性. S-nitroso-N-acetylcysteine (SNAC) 呈现出最高的NO释放和抗菌功效.
科学领域:
- 化学合成 化学合成
- 抗菌剂 抗菌剂 抗菌剂
- 氧化捐赠者 氧化捐赠者
背景情况:
- 氧化 (NO) 在各种生理过程中起着至关重要的作用.
- 开发有效的NO捐赠体对于治疗应用至关重要.
- 研究用于抗菌目的的新型NO供体具有显著的兴趣.
研究的目的:
- 合成和描述四种新的氧化 (NO) 捐赠者:S-酸 (GSNO),S-酸 (CySNO),S-酸 (SNAC) 和2-酸 (GAS).
- 评估这些合成化合物的物理化学特性和NO释放概况.
- 评估NO捐赠者的体外抗菌活性对病原性细菌,特别是大肠杆菌 (大肠杆菌) 和金黄色葡萄球菌 (金黄色葡萄球菌) 的抗菌活性.
主要方法:
- 使用紫外线可见吸收和里埃变换红外光谱学合成和表征四个NO捐赠体.
- 在特定条件下从每个捐赠者释放的氧化 (NO) 的量化.
- 使用抑制区测定对大肠杆菌和黄金杆菌的抗菌活性的评估.
- 使用扫描电子显微镜 (SEM) 检查细菌细胞损伤的显微镜检查.
主要成果:
- 通过光谱分析证实了四个RSNO的成功合成.
- 所有合成的NO捐赠者都不断释放NO,其中SNAC的释放量最高 (高达706.63μmol L-1).
- 所有的NO捐赠者都表现出对大肠杆菌和黄金杆菌的显著抗菌活性,表现优于酸 (NaNO2). 疗效的顺序是SNAC > GSNO > CySNO > GAS > NaNO2. 这一顺序是 SNAC > GSNO > CySNO > GAS > NaNO2.
- SEM分析显示,细菌细胞壁和膜受到不同程度的损伤,大肠杆菌显示出更严重的损伤.
结论:
- 四种新型的氧化 (NO) 捐赠者已成功合成和表征.
- 合成的NO捐赠体显示出对大肠杆菌和黄金色杆菌有强大的抗菌特性.
- 斯纳克 (SNAC) 成为一个特别有效的NO捐赠者,具有卓越的NO释放和抗菌活性.
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