线性ANRIL转录P14AS调节NF-κB信号,以促进结肠癌的进展
1Department of Blood Transfusion, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, 100730, P. R. China.
长线性非编码RNA P14AS通过隔离miR-23a-5p促进结肠癌的进展,导致NF-κB信号的增加. 这种机制突出了P14AS作为结肠癌治疗的潜在治疗标.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 在RNA生物学,RNA生物学.
背景情况:
- 长非编码RNA P14AS,ANRIL的线性转录,在结肠癌中失调.
- 对于P14AS在结肠癌进展中的确切作用尚不清楚.
研究的目的:
- 为了研究P14AS在结肠癌中的调节功能.
- 阐明P14AS影响癌症进展的机制.
主要方法:
- 定量实时聚合酶连锁反应 (qRT-PCR) 和西布洛特测量基因和蛋白质表达.
- 双露西法酶记者测试,RNA免疫沉和拉下测试以确认分子相互作用.
- 细胞计数套件8 (CCK8) 测试以评估细胞增殖和化学敏感性.
主要成果:
- 过度表达P14AS增强了TNF-NF-κB信号传递,增加了结肠癌细胞中IL6和IL8的表达.
- P14AS 直接与 miR-23a-5p 相互作用,该 miR-23a-5p 针对 UBE2D3; UBE2D3 调解 IκBa 降解,增强 NF-κB 信号传递.
- 通过抑制miR-23a-5p或沉默UBE2D3,通过P14AS介导的结肠癌细胞生长促进被废除.
结论:
- 通过隔离miR-23a-5p,P14AS促进结肠癌的进展,从而调节NF-κB信号传递.
- P14AS/miR-23a-5p/UBE2D3轴代表了结肠癌的一个新机制.
- P14AS是结肠癌干预的潜在治疗标.
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