对GATA转录因子及其表达模式在葡萄藤 (Vitis vinifera L.) 的非生物压力下进行分析
Xiuming Zhang1, Jiahui Ma1, Shijin Yang1
1College of Enology and Horticulture, Ningxia University/College of Modern Grape and Wine Industry/Ningxia Grape and Wine Research Institute/Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan, 750021, P. R. China.
BMC plant biology
|December 1, 2023
概括
这项研究确定了葡萄藤中的23个GATA基因,揭示了它们在植物发育和对干旱和寒冷等环境压力的反应中的作用. 这些发现支持未来的葡萄品种育种,以提高耐压力.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 植物生理学 植物生理学
背景情况:
- GATA转录因子对于植物生长和环境反应至关重要.
- 在非生物性压力下对葡萄藤GATA基因家族的研究有限.
研究的目的:
- 鉴定和描述葡萄藤 (Vitis vinifera L.) 中的GATA基因家族.
- 研究VviGATA基因在对非生物压力和发育过程的反应中的潜在作用.
主要方法:
- 遗传学分析来对VviGATA基因进行分类.
- 对基因结构,保存的动机和染色体分布的分析.
- 促进者cis-acting元素分析以预测调节功能.
- 器官/组织表达特征分析和压力治疗实验.
- 选择基因的异质表达和功能分析.
主要成果:
- 确定了23个VviGATA基因,分为四个族系基因组.
- 在群体内发现保存的外子-内子结构和图案,染色体分布不均,表明发生重复事件.
- 促成体分析表明对光,激素和压力的反应.
- 在各种发育阶段和非生物性压力下 (干旱,盐,寒冷) 证明了组织特异性表达和上调.
- 确认了来自V. amurensis的特定VamGATA基因的核定位和转录活性.
结论:
- 提供葡萄藤GATA基因的全面目录.
- 提供了关于VviGATA基因在应激反应和发育中的功能的见解.
- 奠定了利用GATA基因在葡萄树分子育种中用于增强应激弹性的基础.
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