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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
转录的 lncRNA uc.291 在过度增殖性皮肤疾病中的参与
Mara Mancini1,2, Simone Sergio2, Angela Cappello3
1Istituto Dermopatico Dell'Immacolata, IDI-IRCCS, 00167, Rome, Italy.
uc.291转录和ACTL6A的相互作用对于角质细胞的分化至关重要. 这一轴在牛皮中失调,这表明它是皮肤疾病的治疗点.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 角质细胞的分化对于皮肤屏障功能至关重要.
- Uc.291转录通过ACTL6A调节状细胞的分化.
- 不调节的角质细胞分化有助于皮肤多增殖性疾病,如牛皮.
研究的目的:
- 调查uc.291和ACTL6A在牛皮中观察到的失衡的角质细胞分化中的作用.
- 探索uc.291-ACTL6A轴作为牛皮的治疗点的潜力.
主要方法:
- 定量实时PCR (RT-qPCR) 用于评估基因表达.
- 免疫组织化学评估蛋白质水平.
- 对公开可用的数据集进行生物信息分析 (GSE7216).
- 在实验室内类似牛皮的模型使用IL-22治疗的角质细胞.
主要成果:
- 与非病变皮肤相比,牛皮病变皮肤的UC.291,洛里克林 (LOR) 和菲拉格林 (FLG) 表达减少.
- 在牛皮病损伤皮肤中,ACTL6A mRNA和蛋白质水平上调.
- 在体外和体外数据证实了UC.291,FLG和LOR表达的减少,以及在牛皮状状态下ACTL6A表达的增加.
- 治疗IL-22反映了这些表达变化在角质细胞.
结论:
- uc.291转录及其相互作用体ACTL6A在牛皮的发病过程中起着重要作用.
- uc.291-ACTL6A分子轴是牛皮和其他皮肤疾病的潜在治疗点,其特点是差异化受损.
- 该轴的抑制可能为治疗这些疾病提供一种新的药理方法.
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