老化的肌缺乏对急性压力损伤的适应性反应
Samuel J Mlawer1, Eliot H Frank2, Brianne K Connizzo1
1Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
概括
一个新的探索模型显示,急性压缩会损伤肌,老化的肌适应能力降低,损伤风险增加. 年轻肌表现出适应性矩阵周转,与较老肌不同.
科学领域:
- 生物医学工程 生物医学工程
- 肌肉骨生物学 肌肉骨生物学
- 肌病理生理学 肌病理学
背景情况:
- 旋转手套肌病是多因素的,受衰老和压缩的影响.
- 了解肌对这些因素的反应是有限的,而体内模型很难分离这些影响.
- 现有的模型缺乏直接评估肌对机械压缩的反应的能力.
研究的目的:
- 开发和验证一种新型的探索培养模型,用于直接对肌施加压缩.
- 研究年轻和老年肌对急性压力损伤的生物和机械反应.
- 阐明肌适应和受伤反应的年龄相关差异.
主要方法:
- 开发了一种肌膨胀培养系统,可以直接应用压力.
- 对年轻和年长的C57BL/6J flexor digitorum longus肌探头施加一次急性压缩负荷.
- 评估了细胞活力,代谢活性,基因组合,基因表达和机械性质的变化.
主要成果:
- 年轻肌表现出适应性矩阵周转,包括改变的蛋白质甘氨酸含量和增加的原蛋白合成和MMP-9表达.
- 老化的肌表现出代谢活动和原合成的减少,缺乏在年轻肌中看到的适应性反应.
- 急性压缩诱导了肌结构和功能的持久,年龄相关的变化.
结论:
- 这种新型的扩展模型有效地证明了对压缩肌损伤的年龄相关反应.
- 老化的肌具有降低的适应能力,增加了对压缩诱导损伤的易感性.
- 该模型为未来研究在各种负载条件下肌损伤和衰老提供了一个平台.
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