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基于的同步键选择性同位素标记传感器与一次性超微型CARS纤维探测器

Tong Wang, Junfeng Jiang, Kun Liu

|December 2, 2023

概括

这项研究引入了一种新型的一次性纤维探针,用于同时检测使用连贯的反斯托克斯拉曼散射 (CARS) 标记的 (C-D) 和非标记的 (C-H) 键. 这种技术可以实现精确的,选择性键传感,用于代谢跟踪.

科学领域:

生物医学工程生物医学工程
频谱学是一种光谱学.
化学传感器化学传感器

背景情况:

基于的同位素标签对于通过拉曼光谱来跟踪细胞代谢至关重要.
现有的方法往往无法同时检测标记和未标记的债券.
需要敏感,选择性和体内兼容的生物化学检测工具.

研究的目的:

开发和演示一次性超微型纤维探头,用于同时选择性检测低波数 (LW) C-D和高波数 (HW) C-H键.
通过使用标签和连贯的抗斯托克斯拉曼散射 (CARS) 实现代谢过程的实时分析.
为了实现减化合物的定量度测定.

主要方法:

制造一个直径为10.78微米的一次性纤维探针,带有聚焦和时间域走出消除纤维部分.
利用宽频间隔的双Stokes脉冲传递和聚焦,同时激发LW C-D和HW C-H键.
采用连贯的反斯托克斯拉曼散射 (CARS) 光谱技术进行光谱分析.

主要成果:

在混合物样本中成功证明了 LW C-D 和 HW C-H 键的同时检测.
实现了定量度的确定,分辨率低至11mM.
在模拟的细胞环境中,表征了化和非化化合物的化学振动模式.

结论:

一次性CARS纤维探头为体内生化检测提供了一个有前途的新工具.
该探测器能够根据同位素标记进行敏感和选择性传感.
这项技术促进了先进的细胞代谢跟踪和诊断.

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