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一种重组酶聚合酶放大-SYBR绿色I试验,用于快速和可视检测布鲁塞拉菌

Jiang Chang¹, Nan Wang², Jun-Peng Zhan^1,3

¹State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.

Folia microbiologica

|December 2, 2023

概括

一种新的重组酶聚合酶放大-SYBR绿色I (RPAS) 试验允许快速,可视检测布鲁塞拉细菌. 这种用户友好的方法非常适合现场使用,特别是在资源有限的环境中,用于及时预防疾病.

科学领域:

兽医微生物学兽医微生物学
分子诊断学分子诊断
动物传染病研究研究

背景情况:

布鲁塞洛症是一种由布鲁塞拉菌引起的动物传播疾病,对人类健康和动物农业产生重大影响.
传统的布鲁塞拉检测方法耗时且不适合现场监测.
有效的病原体监测对于控制菌病爆发至关重要.

研究的目的:

开发一种快速,可视和可在现场部署的检测方法来检测布鲁塞拉病毒.
针对保存的BCSP31基因进行特定和敏感的病原体识别.
为传统的诊断技术提供一种用户友好的替代方案.

主要方法:

开发一种复合酶聚合酶放大-SYBR绿色I (RPAS) 试验.
针对BCSP31基因,这是布鲁塞拉物种中保存的标记物.
在各种样本类型 (血清,肉类,牛奶) 中验证试验的特异性,灵敏度和性能.

主要成果:

RPAS试验显示高特异性,没有对常见细菌的交叉反应.
检测极限被确定为2.14 × 10^4 CFU/mL或g的布鲁塞拉.
该测量器在20分钟内在40°C有效运行,并且对样品干扰具有坚固耐用性.

结论:

开发的RPAS试验提供了一种快速,视觉直观和用户友好的布鲁塞拉检测方法.
这种分析非常适合资源有限的环境,使得现场监测.
快速检测有助于及时实施预防和控制布鲁塞洛斯病的措施.

关键词:

在BCSP31基因.布鲁塞拉病毒是什么?复合酶聚合酶放大复合酶的放大.在SYBR绿色I上视觉化的可视化

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