用L-氨酸修饰的石墨烯量子点的蓝光驱动光活性及其抗菌作用
Mila Milenković1, Gabriele Ciasca2, Aurelio Bonasera3
1Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, P.O. Box 522, 11000 Belgrade, Serbia.
Journal of photochemistry and photobiology. B, Biology
|December 2, 2023
概括
新的S,N-doped石墨烯量子点 (GQDs) 显示强大的光触发抗菌活性对抗耐药菌株. 马辐射有效地用硫和修改了GQDs,创造了一个有前途的新型抗菌剂.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
背景情况:
- 抗生素耐药性是一个日益严重的全球卫生危机,需要新的抗菌策略.
- 传统的抗生素由于广泛的细菌耐药性而变得越来越无效.
- 迫切需要开发新的抗微生物药物,以对抗耐药细菌感染.
研究的目的:
- 调查硫 (S) 和 (N) 合的石墨烯量子点 (GQD) 作为光触发抗菌剂的抗菌活性.
- 为了合成和表征S,N-化GQDs使用玛辐射和L-氨酸.
- 为了评估这些修改后的GQDs在蓝光暴露下对各种细菌菌株的疗效.
主要方法:
- 石墨烯量子点 (GQDs) 通过在L-氨酸存在的情况下使用玛辐射在单个步骤中进行了修改.
- 用X射线光电谱 (XPS) 和核磁共振 (NMR) 来进行修改的GQD的结构性表征.
- 通过确定在蓝光 (470nm) 照射后对八种细菌菌株的最小抑制度 (MIC) 来评估抗菌活性.
主要成果:
- 马辐射成功地将硫 (1.14at.%) 和 (1.81at.%) 引入了GQD结构,显著改变了sp2碳域.
- 经过修改的GQD显示出结合人类血清白蛋白的能力.
- 在S,N-doped的GQD样本 (GQD-cys-25) 显示出显著的抗菌活性,最低抑制度 (MIC) 为125μg/mL对*S. aureus*,*E. faecalis*和*E. coli*在1小时的蓝光暴露后.
结论:
- 通过玛辐射合成的S,N-化GQDs代表了一种新且有效的光触发抗菌剂.
- 修改过程成功地引入了异质原子,并改变了GQD的电子特性,增强了它们的抗菌潜力.
- 这些发现表明,开发先进的抗微生物材料来打击抗生素耐药性的有希望的新途径.
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