在体内表达的真菌细菌转录作为肺结核病的诊断标
Sumedha Sharma1, Ashutosh N Aggarwal2, Prabhdeep Kaur1
1Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, 160012, India.
Tuberculosis (Edinburgh, Scotland)
|December 2, 2023
概括
来自体内表达的真菌细菌转录的新型生物标志物显示出诊断肺结核 (PTB) 的前景. 中的Rv0986,Rv0971c和Rv3121基因的实时PCR分析为PTB提供了一种新的诊断方法.
科学领域:
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
- 生物标志物发现发现
背景情况:
- 像Xpert MTB/RIF这样的核酸放大测试 (NAAT) 已经改善了结核病 (TB) 的诊断.
- 为了实现消除结核病的全球目标,新的诊断测试是必不可少的.
- 目前的诊断需要加强,以早期和准确地检测肺结核 (PTB).
研究的目的:
- 调查PTB诊断中体内表达的真菌细菌转录生物标志物的潜力.
- 使用实时PCR评估特定基因转录 (Rv0986,Rv0971c,Rv3121) 的诊断准确性.
- 评估这些生物标志物的实用性,无论是涂抹阳性还是涂抹阴性PTB病例.
主要方法:
- 招募了61名受试者:15名涂抹阳性PTB,30名涂抹阴性PTB和16名疾病对照.
- 在唾液样本中分析菌根细菌基因转录Rv0986,Rv0971c和Rv3121.
- 使用定量实时PCR (qRT-PCR) 量化转录水平的量化.
主要成果:
- 对于Rv0986,Rv0971c和Rv3121的qRT-PCR显示,在涂抹阳性PTB患者中,分别具有100%,78.6%和86.7%的灵敏度.
- 在涂抹阴性PTB患者中,Rv0986和Rv0971c显示出63%的灵敏度,而Rv3121显示出~40%的灵敏度.
- 对所有三种基因的组合分析在涂抹阴性PTB患者中增加了对85%的敏感性.
结论:
- 在体内表达的真菌细菌转录是PTB诊断的有希望的生物标志物.
- 转录组方法为改善PTB检测提供了一种新的策略,特别是在涂抹阴性病例中.
- 这些生物标志物的进一步验证可能会导致改善结核病的诊断工具.
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