相关实验视频

Updated: Jul 9, 2025

Shotgun Lipidomics of Rodent Tissues

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

转录组和脂质组的整合揭示了在Shitou中脂肪肝形成的机制

Longsheng Hong¹, Zongyi Sun², Danning Xu²

¹College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.

Poultry science

|December 2, 2023

概括

什图在过度养时会产生脂肪肝,其标志是肝脏膨胀和甘油三水平升高. 诸如不和脂肪酸生物合成和脂质代谢等关键途径都与这种情况有关.

科学领域:

动物科学动物科学
生物化学生物化学
基因组学就是基因组学.

背景情况:

作为迁徙鸟类的,容易将高能量的料转化为甘油三.
过度的甘油三沉积会导致脂肪酸氧化不完全和脂质积累,导致脂肪肝.
中国的什图通过24小时的棕过度养,独特地发展出脂肪肝.

研究的目的:

研究在被过度养的西图中脂肪肝形成背后的分子机制.
确定关键的基因,代谢物和信号通路,参与饮食诱导脂肪肝的发展.

主要方法:

从正常养 (对照) 和过度养的西图收集和分析肝脏样本.
进行生物化学分析以测量肝脏大小,脂质含量和肝脏损伤指标 (ALT,AST).
进行了组合的转录基因和脂质基因分析,以确定差异表达基因 (DEG) 和显著差异丰富的代谢物 (SDM).

主要成果:

过度养的表现出明显更大的肝脏 (3.5倍重) 与广泛的脂质滴透.
生物化学分析显示过度养的肝脏总胆固醇,甘油三和糖原水平升高,ALT或AST没有显著变化.
转录基因和脂质基因分析确定了1510个DEG和1559个SDM,在代谢和脂质途径中丰富.

结论:

确定了三个关键的信号通路:不和脂肪酸的生物合成,糖脂代谢和糖脂代谢.
特定的基因 (例如GPAM,AGPAT2,DGAT2) 和代谢物 (例如TG,PA,PE) 被突出显示为可能调节TG合成并促进脂肪肝的进展.
这项研究提供了关于Shitou的饮食诱导脂肪肝的遗传和代谢调节的见解.

关键词:

什图的子什图的子脂肪肝是一种脂肪肝.脂质组 (lipidome) 是一种脂质组 (lipidome) 的组成部分.转录组 (transcriptome) 是一个转录组.三甘油的积累是因为三甘油的积累.

更多相关视频

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

Published on: March 10, 2023

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Lipid Digestion

Lipid Digestion

Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.