自调节改变了T24膀癌细胞的机械化学敏感性
Maximilian Jobst1, Maliha Hossain2, Endre Kiss3
1Department of Food Chemistry and Toxicology, University of Vienna Faculty of Chemistry, Währinger Str. 38-40, 1090 Vienna, Austria; Core Facility Multimodal Imaging, University of Vienna Faculty of Chemistry, Währinger Str. 38-40, 1090 Vienna, Austria; University of Vienna, Vienna Doctoral School in Chemistry (DoSChem), Währinger Str. 42, 1090 Vienna, Austria.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
|December 2, 2023
概括
研究了诸如巴菲洛米辛和沃特曼宁等天然化合物,以及菌毒素脱氧尼瓦伦醇和酸,以了解它们对膀癌细胞适应性的影响. 这些化合物影响细胞行为和迁移,为癌症治疗研究提供了潜在的新途径.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 膀癌细胞适应环境的化学和机械线索,可能导致化疗耐药性.
- 自在癌细胞的适应和抵抗中起着重要作用.
- 包括真菌代谢物和真菌毒素在内的天然化合物正在研究它们在癌症中的治疗潜力.
研究的目的:
- 研究由真菌衍生的自抑制剂 (巴菲洛米辛,沃特曼宁) 和真菌毒素 (脱氧尼瓦伦醇,酸) 调节膀癌细胞适应性的潜力.
- 评估这些化合物对细胞形态,迁移和对机械刺激的反应的影响.
- 探索PIEZO通道在调解这些化合物对癌细胞影响中的作用.
主要方法:
- 对膀癌细胞施加了巴菲洛米辛 (BAFI),沃特曼宁 (WORT),脱氧尼瓦伦醇 (DON) 和酸 (FA) 的非细胞毒性度.
- 分析了细胞形态和细胞迁移.
- 研究了剪切应力和PIEZO通道抑制对BAFI和FA细胞反应的影响.
- 测试了具有类似细胞毒性潜力的化合物的组合.
主要成果:
- 在非细胞毒性度下,BAFI和WORT改变了细胞形态和减少了迁移.
- 剪切应力和PIEZO通道抑制改变了细胞对BAFI的敏感性.
- FA对细胞运动的调节作用与PIEZO1的表达和抑制有关.
- 不同的化合物组合在细胞机械转导上表现出不同的影响,尽管细胞毒性相似.
结论:
- 菌代谢物和菌毒素可以显著影响膀癌细胞的适应性和机械传导.
- 皮佐通道参与调解某些化合物对癌细胞运动性的作用.
- 这些发现为化学抵抗和相关途径提供了新的视角,突出了天然化合物在癌症研究中的潜力.
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