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使用基于数字微流体的光 in situ 杂交平台检测EGFR基因放大

Chuanjie Shen¹, Cheng Zhan², Zhaoduo Tong¹

¹State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China.

|December 2, 2023

概括

一个新的基于数字微流体的光在位混合 (DMF-FISH) 平台自动检测EGFR放大非小细胞肺癌 (NSCLC). 这种自动化系统提供与传统方法相当的性能,同时显著降低了探头消耗.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断
在瘤学瘤学.

背景情况:

皮表皮生长因子受体 (EGFR) 基因信号传递对瘤进展至关重要.
在非小细胞肺癌 (NSCLC) 中EGFR放大预测对氨酸激酶抑制剂的敏感性.
传统的光现场杂交 (FISH) 用于EGFR放大是劳动密集型和耗时的.

研究的目的:

开发和验证一个完全自动化的基于微流体的数字FISH (DMF-FISH) 平台.
为了简化在NSCLC中检测EGFR放大.
为了减少FISH试验的复杂性和资源需求.

主要方法:

开发一个DMF-FISH系统,集成DMF芯片,加热阵列和信号处理.
自动执行FISH步骤,包括细胞消化,杂交和染色.
用细胞系和临床样本测试DMF-FISH平台.

主要成果:

在没有人工干预的情况下,DMF-FISH系统成功地自动化了FISH操作.
该平台的诊断性能与传统的基板 FISH.相提并论.
与传统方法相比,DMF-FISH 减少了 87% 的DNA 探针消耗.

结论:

自动化DMF-FISH平台为EGFR放大检测提供了一个可行的和高效的替代方案.
这项技术在改善NSCLC的临床诊断和个性化治疗方面具有显著的潜力.
DMF-FISH解决了传统FISH的局限性,提高了可访问性和降低了成本.

关键词:

数字微流体学数字微流体学欧洲农业基准基金放大功率光在现场混合化中的光.

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相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...