工程大肠杆菌Escherichia coli以经济高效的生产中链脂肪酸从大豆乳清使用优化的银河糖基自诱导系统
Zhe Wang1, Yiqiang Dai1, Fidelis Azi2
1Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Bioresource technology
|December 2, 2023
概括
这项研究开发了一种具有成本效益的E.coli平台,用于使用大豆乳清和银河糖生产中链脂肪酸 (MCFA). 这种方法提高了微生物生产效率,并降低了有价值的化学原料的成本.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 中链脂肪酸 (MCFA) 是重要的化学原料,微生物生产为传统方法提供了可持续的替代方案.
- 目前的微生物生产受到昂贵的介质和外部诱导因素的阻碍,限制了经济可行性.
- 开发具有成本效益的微生物平台对于广泛的MCFA应用至关重要.
研究的目的:
- 为MCFA生产设计一个经济的E.coli平台.
- 为了利用大豆乳清作为低成本的介质和银河糖作为自我诱导剂.
- 通过代谢工程策略来提高MCFA产量.
主要方法:
- 通过修改GalP促进剂以进行严格的酶表达,设计了一种强大的基于银河糖的自诱导系统.
- 增强细胞内乙-CoA的可用性和NADH的再生,以优化β氧化循环的逆转.
- 使用pH控制的批量和料批量发酵策略与大豆乳清介质.
主要成果:
- 在批发发酵中获得的MCFA产量为8.20 g/L,在料批发发酵中达到16.42 g/L.
- 证明了大豆乳清作为成本效益高的介质的有效性.
- 成功实施了银河糖自诱导用于酶表达路径.
结论:
- 提出了一个具有成本效益和有前途的E.coli平台用于MCFA生产.
- 开发的系统为工业MCFA合成提供了一个可扩展的解决方案.
- 为未来针对其他高价值化学品的菌株开发提供了基础.
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