通过工程Pichia pastoris在糖养批发发酵中使用高效的透系统进行高水平的醇生物生产
Ryota Kumokita1, Takahiro Bamba2, Hisashi Yasueda3
1Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Bioresource technology
|December 2, 2023
概括
研究人员设计了Pichia pastoris酵母,从甘油中产生高水平的生物生产. 一个新的引系统显著提高了的产量,达到3304毫克/升,克服了毒性挑战.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 微生物生产 微生物生产
背景情况:
- 是一种关键的工业化学品,具有有限的可持续生产方法.
- 由于细胞抑制,微生物产生像这样的有毒化合物具有挑战性.
- 皮奇亚牧师 (Komagataella phaffii) 是一个强大的酵母平台,用于生物技术应用.
研究的目的:
- 在Pichia pastoris.中开发一个高水平的醇生物生产系统.
- 为了克服酵母发酵中的毒性限制.
- 建立一个可扩展的微生物过程,用于合成.
主要方法:
- 通过引入氨酸醇酶,对Pichia pastoris进行代谢工程.
- 开发了一种Pichia pastoris菌株,该菌株过度生产氨酸,这是一个醇前体.
- 使用空心纤维膜接触器和支柱膜的透系统的实施.
- 糖醇料批发发酵与引系统集成.
主要成果:
- 在初始瓶培养中达到59 mg/L的.
- 在用甘油养的批发发酵中达到1052mg/L的.
- 已证明的度 >1000 mg/L 抑制了酵母菌的生长.
- 综合透系统使醇度增加了214%,达到3304 mg/L.
结论:
- 代谢工程和过程优化使Pichia pastoris.中的高度醇生产成为可能.
- 开发的引系统有效地减轻了毒性,增强了微生物的生长.
- 这项研究为微生物生产有毒化学品和材料提供了一个框架.
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