同时的中链碳酸和1,3-二醇在生物增强的乳酸基链延长中产生的中链碳酸和1,3-二醇
Anna Duber1, Roman Zagrodnik2, Wojciech Juzwa3
1Water Supply and Bioeconomy Division, Faculty of Environmental Engineering and Energy, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
Bioresource technology
|December 2, 2023
概括
在开放发酵中,生物增量增强了链条延长. 特定的共同培养和基质,如甘油,促进了的生产,而Eubacterium limosum则促进了有价值的奇偶碳产品.
科学领域:
- 微生物的新陈代谢和发酵过程
- 生物技术和生物工艺工程
- 合成生物学 合成生物学
背景情况:
- 开放培养发酵为生产有价值的化学品提供了一种经济有效的方法.
- 生物增量可能会在复杂的环境中提高微生物的性能和产品选择性.
- 控制微生物联盟对于优化发酵结果至关重要.
研究的目的:
- 为了研究生物增量对链延长的影响,使用糖醇,乳酸盐和乳糖作为基质.
- 评估特定微生物菌株 (Megasphaera elsdenii, Eubacterium limosum) 在开放发酵中的生存和性能.
- 了解不同基质和发酵模式 (批次与连续) 对产品形成的影响.
主要方法:
- 使用甘油,乳酸盐和乳糖进行开放培养发酵实验.
- 用Megasphaera elsdenii和Eubacterium limosum的共同培养进行生物增量.
- 发酵产品的分析,包括酸,1,3-二醇 (1,3-PDO) 和短链碳酸盐.
- 图像流细胞计和机器学习用于微生物细胞歧视的应用.
主要成果:
- 在糖醇上的M. elsdenii和E. limosum的共同培养中实现了最高的鱼选择性 (28.6%).
- 利莫苏姆菌 (Eubacterium limosum) 证明了生存,并促进了奇偶碳链的延长和酸盐的产生.
- 糖醇的连续发酵产生了酸和1,3-PDO;乳酸盐的添加产生了1,3-PDO和短链碳酸.
- 批量模式发酵有利于偶碳链延长.
结论:
- 使用特定菌株 (如E. limosum) 的生物增量可以显著提高开放发酵中的链延长效率和产品配置.
- 基质的选择和发酵模式对代谢途径和最终产品具有重要影响.
- 像成像流细胞计和机器学习这样的综合技术对于在复杂的发酵过程中监测微生物种群是有效的.
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