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Updated: Jul 9, 2025

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在哺乳动物细胞中可编程的 de novo 设计的卷状卷状介导相分离

Maruša Ramšak1,2, Dominique A Ramirez3, Loren E Hough4

  • 1Department of Synthetic Biology and Immunology, National Institute of Chemistry, Ljubljana, Slovenia.

Nature communications
|December 2, 2023
PubMed
概括

研究人员设计了新的可调节模块,用于在细胞中创建无膜液体隔间或液态液相分离 (LLPS) 凝聚物. 这种模块化方法为理解和控制生物过程提供了一个平台.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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