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一种新型MgtE同类物及其在膜模拟物中的结构动态的表征

Rupasree Brahma¹, H Raghuraman¹

¹Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, India; Homi Bhabha National Institute, Training School Complex, Mumbai, India.

Biophysical journal

|December 3, 2023

概括

这项研究揭示了细菌转运器 (MgtE) 在没有其典型的传感域的情况下如何运作. 它突出显示了传送器.

科学领域:

生物化学生物化学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

离子 (Mg2+) 是细胞必不可少的组成部分,但细胞内运输机制尚不清楚.
MgtE蛋白家族促进了细菌中的Mg2+运输,并与与疾病相关的人类载体具有同质性.
在生物相关的膜环境中,MgtE的结构数据有限.

研究的目的:

描述Bacillus firmus MgtE (MgtE_BF) 的结构和功能,这是一个缺乏Mg2+感应N域的独特同类物.
在功能性膜背景下研究MgtE_BF的Mg2+结合和运输能力.
探索膜仿真中MgtE_BF与关相关的动态.

主要方法:

蛋白质的净化和功能性复制成蛋白质体.
ColabFold用于结构预测.
微尺度热泳用于结合性研究.
在膜仿真中分析Trp残留物动态.

主要成果:

MgtE_BF被净化,并预测形成一个同位素.
MgtE_BF结合了Mg2+和ATP. 这两种化合物.
尽管缺乏N域,MgtE_BF在蛋白质体中调解Mg2+运输.
结合Mg2+会在跨膜领域引起微妙的形状变化.

结论:

MgtE_BF表明Mg2+运输活动独立于其N域.
脂质-蛋白质相互作用在与原生环境相似的环境中显著影响MgtE门机制.
这项工作提供了对Mg2+运输调节和潜在治疗点的见解.

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