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Receptor Tyrosine Kinases

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

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Updated: Jul 9, 2025

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

通过基质相互作用和酸化来表征细胞外c-Src氨酸激酶功能的协议.

Michael P Omini¹, Deema Alsalih², SarahBeth D Votra²

¹Department of Urology, SUNY Upstate Medical University, Syracuse, NY 13210, USA; Department of Biology, College of Arts and Science at Syracuse University, Syracuse, NY 13244, USA.

|December 3, 2023

概括

这项研究引入了一项新协议,用于识别癌细胞分泌的细胞外氨酸酸化基质. 这种方法有助于理解细胞外Src (e-Src) 在癌症进展中的作用.

关键词:

癌症癌症癌症癌症细胞生物学细胞生物学分子生物学分子生物学

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Published on: June 30, 2019

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Last Updated: Jul 9, 2025

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Identification of Kinase-substrate Pairs Using High Throughput Screening

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Published on: August 29, 2015

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Published on: June 30, 2019

科学领域:

生物化学生物化学
细胞生物学细胞生物学
在瘤学瘤学.

背景情况:

细胞Src氨酸激酶 (c-Src) 在人类癌症的秘密体中发现,称为细胞外Src (e-Src).
蛋白学确定了114种潜在的细胞外e-Src基质,包括金属蛋白酶的组织抑制剂 2.

研究的目的:

介绍一个详细的协议,以表征由c-Src表达和分泌产生的分泌的氨酸酸化基质.
为研究其他细胞外激酶提供一种可适应的方法.

主要方法:

细胞秘密和提取物的收集.
抗体治疗和Ni-NTA拉下测试.
检测蛋白质-蛋白质相互作用和基质的氨酸酸化.

主要成果:

描述了一个全面的协议,用于识别c-Src.的细胞外基质.
该方法允许在细胞外环境中表征氨酸酸化蛋白质.
该协议有助于研究e-Src在癌症分泌体中的作用.

结论:

开发的协议使得与c-Src.相关的分泌氨酸酸化基质的表征成为可能.
这种方法对于研究细胞外激酶在各种生物环境中的功能,特别是在癌症研究中,非常有价值.
该协议为未来研究细胞外激酶信号通路提供了基础.