相关实验视频

Updated: Jul 9, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

抗生素耐药性基因在塑层的动态演变在城市河流的垂直轮中

Yufang Chen¹, Zhenhua Yan¹, Yixin Zhou²

¹Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes of Ministry of Education, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.

|December 3, 2023

概括

微塑料 (MP) 作为抗生素耐药性基因 (ARG) 的热点. 这项研究揭示了水深对河流塑层中的ARG有重大影响,影响了它们的传播和转移动态.

科学领域:

环境科学环境科学
微生物学微生物学
环境化学环境化学

背景情况:

微塑料 (MPs) 在水生环境中垂直运输,形成不同的塑料球.
众议院被认为是抗生素耐药性基因 (ARG) 的热点,但它们在塑球中的垂直传播和转移仍未得到充分研究.
鉴于全球PM污染日益增加,了解塑料球中的ARG动态至关重要.

研究的目的:

研究在城市化河流中垂直分布的不同塑球中ARG的动态反应和演变.
为了确定水深和MP类型对ARG丰度和分布的影响.
探索微生物群落,营养素和塑料球中的ARG传播之间的关系.

主要方法:

在不同水深的聚乳酸 (PLA) 和聚乙烯四甲酸 (PET) 塑料球中分析生物膜生物质量和ARG丰度.
在塑球中对ARG丰度动态进行时间监测.
微生物社区分析和与营养含量和移动遗传元素 (MGE) 的相关性.

主要成果:

塑圈的ARG含量明显高于周围水域,PLA和PET之间有显著的差异.
随着水深的变化,ARG的丰富度随着水深的变化而变化,在PET塑料球中深度随着深度的增加,而PLA在中间水中显示出丰富度的峰值.
深水塑球表现出更丰富的微生物群落和复杂的相互作用,与营养含量相关,并促进ARG传播和水平基因转移.

结论:

水深是影响河流塑层内ARG传播和转移的关键因素.
塑圈社区的组成和不同水层中营养的可用性推动了ARG的进化.
调查结果强调了在评估MP和相关ARG的生态风险时考虑垂直配置文件的重要性.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.生物膜是一种生物膜.横向的基因转移是可以实现的.微生物群中的微生物群.垂直分布垂直分布的分布.

更多相关视频

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

相关概念视频

Antibiotic Selection

Antibiotic Selection

Genomic DNA in Prokaryotes

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...

Genome Size and the Evolution of New Genes

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.