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细胞外蛋白质增强了Cupriavidus pauculus的耐受性和细胞聚合物的形成

Mingwei Wang¹, Christel Vollstedt², Bente Siebels³

¹School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; Department of Microbiology and Biotechnology, University of Hamburg, Hamburg 22609, Germany.

Bioresource technology

|December 3, 2023

概括

细胞外蛋白 (e-PNs) 对于像Cuprividus pauculus这样的细菌的重金属耐药性至关重要. 这些蛋白质,特别是含有托的蛋白质,有助于离子 (Ni2+) 吸附和细胞防御机制.

科学领域:

微生物学微生物学
环境科学环境科学
生物化学生物化学

背景情况:

耐重金属细菌产生细胞外蛋白质 (e-PNs).
在重金属阻力中e-PNs的特定功能尚未得到充分理解.
细菌中离子 (Ni2+) 耐药性是环境关注的一个重要领域.

研究的目的:

阐明细胞外蛋白 (e-PNs) 在细菌对离子 (Ni2+) 耐药性的作用.
为了确定特定的分子组件和代谢途径,涉及到Cuprividus pauculus中的Ni2+耐药性.
为电子PNs对重金属吸附和抵抗的贡献开发一个概念模型.

主要方法:

里埃变换红外光谱法 (FTIR) 用于分析蛋白质的功能组.
蛋白酶K治疗,以评估e-PNs的必要性.
3D激发发射矩阵光谱学测量托蛋白质的数量.
蛋白质组学和定量实时聚合酶链反应 (qRT-PCR) 用于代谢分析.

主要成果:

FTIR确定了N-H,C=O和NH2-R组对于Ni2+吸附和抵抗至关重要.
蛋白酶K治疗显著降低了Ni2+耐药性,证实了e-PNs的重要作用.
在Ni2+暴露时,e-PNs中的托芬蛋白增加.
尼2+治疗诱导了显著的代谢变化,包括脂多糖生物合成,分泌途径,尼2+运输体和多药物排泄系统的改变.

结论:

细胞外蛋白 (e-PNs) 对于细菌的Ni2+抵抗和吸附至关重要.
特定的蛋白质成分和代谢途径是细菌在Ni2+污染环境中生存的关键.
这项研究为了解通过e-PNs调解的细菌重金属耐药机制提供了一个框架.

关键词:

流量的流量.重金属的阻力耐重金属的阻力一个秘密,一个秘密.

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