有效成分组合通过在COPD中唤起巨细胞自来缓解PM2.5诱导的炎症
Jing Wang1, Weijing He2, Huiyu Yue2
1Henan Key Laboratory of Chinese Medicine for Respiratory Disease, Henan University of Chinese Medicine, Zhengzhou, Henan Province, 450046, China; Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of PR China, Zhengzhou, Henan Province, 450046, China; Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450000, China.
来自Bufei Yishen配方 (BYF) 的有效成分兼容性 (ECC) 通过增强自和激活Foxo3信号来减少慢性阻塞性肺病 (COPD) 的炎症. 这种治疗可以保护大鼠和细胞免受PM2.5暴露.
科学领域:
- 传统中国医药 传统中国医药
- 肺部病理学 肺部病理学
- 细胞生物学 细胞生物学
背景情况:
- 布菲·伊申配方 (BYF) 治疗慢性阻塞性肺部疾病 (COPD).
- 来自BYF的有效组件兼容性 (ECC) 显示了对COPD的同等有效性,并防止PM2.5暴露.
- 需要阐明ECC的保护作用的精确机制.
研究的目的:
- 研究ECC-BYF对PM2.5加速性慢性肺炎的抗炎作用的机制.
- 探索自和Foxo3信号在ECC-BYF的作用中的作用.
主要方法:
- 在体外:用PM2.5刺激MH-S巨细胞;评估自标志物 (西方斑点,免疫光) 和炎症类细胞因子 (ELISA,qPCR).
- 在体内:PM2.5加速的COPD大鼠模型;评估了肺功能,病理,自和炎症媒介.
- 研究了对ECC-BYF效应的自抑制和Foxo3敲击作用.
主要成果:
- 暴露于PM2.5增加了巨细胞,降低了自细胞流量,并在老鼠和细胞中增加了炎症.
- ECC-BYF抑制了PM2.5诱导的炎症,增强了自流,并增加了MH-S细胞中的Foxo3表达和核转位.
- 在COPD大鼠中,ECC-BYF治疗减轻了自失调,增加了FOXO3,减少了肺炎,改善了肺功能.
结论:
- 在COPD中,ECC-BYF改善了PM2.5加重的炎症.
- 该机制涉及通过Foxo3激活在膜巨细胞中增强自流.
- ECC-BYF证明了因PM2.5暴露而加剧的COPD的治疗潜力.
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