在兴奋剂分析中使用QuEChERS作为替代提取程序
Daniel Derwand1,2, Sebastian Rzeppa1, Sven C Voss1
1Institute of Doping Analysis and Sports Biochemistry Dresden, Kreischa, Germany.
Drug testing and analysis
|December 3, 2023
概括
一种新的QuEChERS方法用于用于兴奋剂控制的尿样准备,有效提取312种化合物. 这种具有成本效益的技术,使用酸和酸,符合世界反兴奋剂机构检测禁止物质的要求.
科学领域:
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
- 生物化学 生物化学
背景情况:
- QuEChERS (快速,轻松,廉价,坚固和安全) 是食品分析中广泛使用的样品制备技术,用于检测农药和真菌毒素等残留物.
- 传统的兴奋剂检测样本准备方法可能耗时且昂贵.
- 世界反兴奋剂机构 (WADA) 对用于兴奋剂控制的分析方法制定了严格的性能要求.
研究的目的:
- 评估一种基于QuEChERS的方法,用于准备用于兴奋剂控制的尿样.
- 为了评估一个特定的QuEChERS协议 (酸/酸在酸中) 提取广泛的兴奋剂的效率.
- 根据WADA对确认程序的要求验证该方法.
主要方法:
- 开发了一种QuEChERS方法,使用10M酸和3%的酸在酸中进行尿液样本的制备.
- 该方法在不同pH值和特异性重度的尿样上进行了测试.
- 对342种化合物的提取效率进行了评估,重点关注9种代表WADA禁止清单六个类别的选定分析物.
主要成果:
- 优化的QuEChERS方法成功提取了342个测试化合物中的312个,它们的最低要求性能水平.
- 该方法在提取9个选定的分析物中表现出高效率,符合WADA确认程序的标准.
- 特别关注的是完整的 stanozolol-glucuronides 的提取,显示了高分辨率质谱分析的有希望的结果.
结论:
- 开发的QuEChERS方法提供了一种时间和成本效益的替代方案,用于在兴奋剂控制中准备尿样.
- 这种方法可以成为一种有价值的工具,用于确认非值禁止物质的分析.
- 该方法的效率和符合WADA标准,使其成为反兴奋剂实验室的可行选择.
更多相关视频
04:17Analysis of Organochlorine Pesticides in a Soil Sample by a Modified QuEChERS Approach Using Ammonium Formate
Published on: January 20, 2023
3.2K
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.7K
相关概念视频
01:30Capillary Electrophoresis: Applications
401
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
401
01:05High-Performance Liquid Chromatography: Elution Process
492
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
492
00:56Extraction: Advanced Methods
450
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
450
01:09Ion-Exchange Chromatography
532
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
532