使用LC-MS从OMW提取的化合物的识别
Hanna Fattoum1,2, Aicha O Cherif1, Sameh Trabelsi3
1Laboratory Materials Molecules and Applications LR11ES22, University of Carthage.
Journal of oleo science
|December 3, 2023
概括
橄厂废水 (OMW) 含有高的有机物质. 这项研究发现,以乙酸乙烯的液体-液体提取最有效地提取化合物和增值OMW,根据型的不同抗氧化活性.
科学领域:
- 环境化学环境化学
- 农业科学 农业科学
- 食品化学 食品化学
背景情况:
- 橄厂废水 (OMW) 是橄油生产的主要污染物,因为其有机物质含量很高.
- 对OMW的价值化对于减轻环境影响和探索潜在的资源回收至关重要.
- OMW中的化合物有助于其生物活性和潜在的应用.
研究的目的:
- 通过提取化合物来研究OMW的价值化.
- 评估不同提取方法和溶剂对含量和抗氧化能力的影响.
- 为了识别OMW提取物中的型和它们与抗氧化活性的相关性.
主要方法:
- 使用乙烯酸乙烯的液体-液体提取.
- 使用甲/甲醇 (1:1) 溶剂混合物进行化.
- 使用ABTS测试进行抗氧化活性评估.
- 使用超高性能液体染色学-质谱学 (UHPLC-MS) 进行化合物分析.
主要成果:
- 使用乙酸乙烯的液-液提取产生了最高的多醇含量 (1.17 g GAE/L).
- 用甲/甲醇进行化,可达到1.07g GAE/L的多醇含量.
- 甲醇提取物通过ABTS测定表现出最强的抗氧化活性 (15.75 mg/L).
- 在UHPLC-MS分析中,不同提取物中发现了不同的形状,这表明化合物度和抗氧化活性之间存在复杂的关系.
结论:
- 乙酸乙烯液体液体提取是OMW价值化的有效方法,最大限度地提高了化合物回收率.
- OMW 提取物的抗氧化活性受特定的形状的影响,而不仅仅是总含量.
- 需要对已确定的型进行进一步的研究,以充分了解它们对抗氧化剂潜力和潜在应用的贡献.
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