生物灵感微反应器从CO2中持续合成葡萄糖前体,其能量转换效率是大米的3.3倍
Yujiao Zhu1,2,3, Fengjia Xie1,3, Tommy Ching Kit Wun4
1Department of Applied Physics, The Hong Kong Polytechnic University, Kowloon, Hong Kong, 999077, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|December 3, 2023
概括
这项研究开发了一种模仿植物甲状腺素的人工光合作用反应器,将二氧化碳转化为葡萄糖前体. 这种新型反应器显著提高了酶的效率,稳定性和可重复使用性,为粮食安全和碳中和提供了有希望的解决方案.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 人工光合作用的人工光合作用
背景情况:
- 过度的大气二氧化碳和粮食短缺是全球面临的关键挑战.
- 在将二氧化碳转化为食物的自然光合作用过程中,由于RuBisCO酶的限制,其能量效率较低.
- 以前的努力集中在酶工程上,但这项研究探索了反应堆设计以提高效率.
研究的目的:
- 设计和开发一种新的微反应器,模仿叶绿体的甲状腺体结构.
- 为了使酶D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) 固定,以提高二氧化碳的转化.
- 为了提高RuBisCO的效率,稳定性和可重复使用性,用于人工光合作用.
主要方法:
- 利用层层的策略将RuBisCO固定在微反应器中,模仿甲状腺蛋白堆叠.
- 测量了二氧化碳持续转化为葡萄糖前体的速度.
- 与免费的RuBisCO相比,评估了酶活性,稳定性和可重复使用性.
- 并行调整微型反应器系统的规模,以评估生产能力和能量转换效率.
主要成果:
- 在1.9nmolmin-1.1时实现了持续的CO2转化为葡萄糖前体.
- 与自由RuBisCO.相比,酶活性增加了1.5倍,稳定性增加了大约8倍.
- 报告了固定酶的高可重复使用性,在10个重复使用周期后96%的保留率.
- 扩大规模的反应堆实现了15.8nmolmin-1的生产率,能量转换效率是大米的3.3倍.
结论:
- 这种新型的甲状腺模拟微反应器有效地提高了RuBisCO在二氧化碳转化方面的性能.
- 这种人工光合作用系统与自然光合作用相比,具有更高的能量转化效率.
- 开发的技术显示出大量生产的巨大潜力,有助于粮食供应和碳中和努力.
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