RNF183通过脂肪酸氧化促进结肠癌细胞干细胞的形成
Yingming Song1, Xiaolin Li2, Huiping Wu3
1Department of Gastrointestinal Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi, China.
Nutrition and cancer
|December 4, 2023
概括
通过增强脂肪酸氧化,RNF183促进结肠癌的生长. 抑制这一过程可能为结肠癌 (COAD) 提供一种新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- 结肠癌 (COAD) 是一种主要的胃肠道恶性瘤,其特征是癌症干细胞 (CSC).
- RNF183与结直肠癌转移有关,但其在COAD干系中的作用仍未确定.
- 了解RNF183在COAD干性中的功能对于开发向疗法至关重要.
研究的目的:
- 为了研究RNF183在结肠癌干的作用.
- 探索RNF183和COAD中的脂肪酸氧化之间的关系.
- 评估RNF183作为结肠癌的潜在治疗标.
主要方法:
- 在COAD中对RNF183表达和通路的生物信息分析.
- 免疫组织化学 (IHC) 用于RNF183蛋白检测.
- 流细胞计分离CD133+CD44+CSCs. 这种流细胞计分离CD133+CD44+CSCs.
- 基因和蛋白质表达分析的qPCR,西斑和免疫光.
- 细胞活力 (CCK-8) 和球体形成测试.
- 用Orlistat进行的脂肪酸氧化测定和抑制研究.
主要成果:
- 在COAD组织和CSC (CD133+CD44+) 中,RNF183的表达很高.
- RNF183表达与脂肪酸氧化基因正相关,并促进了COAD干性.
- 在脂肪酸代谢途径中观察到RNF183的丰富.
- 奥尔利斯塔特治疗逆转了RNF183过度表达的亲干效应.
结论:
- RNF183促进结肠癌的生长和脂肪酸的氧化.
- RNF183的机制涉及调节脂肪酸氧化.
- RNF183代表了抑制结肠癌进展的潜在治疗标.
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