再生赋予转录因子复合体ERF115-PAT1协调了根结线虫诱导的胆汁中受伤诱导的反应
Cleberson Ribeiro1,2, Bruno Paes de Melo1,2,3, Isabela Tristan Lourenço-Tessutti1,3
1INRAE, Université Côte d'Azur, CNRS, ISA, Sophia Antipolis, 06903, France.
The New phytologist
|December 4, 2023
概括
根结线虫 (RKN) 通过伤口愈合反应诱导胆结. ERF115-PAT1复合体协调应激信号和组织再生,这对胆汁发育和线虫繁殖至关重要.
科学领域:
- 植物病理学 植物病理学
- 分子植物微生物相互作用.
- 植物开发 植物开发
背景情况:
- 根结线虫 (RKN) 诱导宿主植物根部的特征性胆汁,这一过程假定涉及受伤诱导的再生.
- 众所周知,ERF115-PAT1转录因子复合体能够调节植物中受伤诱导的再生.
研究的目的:
- 研究ERF115-PAT1复合体在RKN胆汁形成和发育中的作用.
- 阐明植物细胞对RKN寄生虫的反应和启动胆汁发育的分子机制.
主要方法:
- 在Arabidopsis thaliana的RKN感染期间,可视化体力压力的对焦成像.
- 使用ERF115和PAT1.1的宫外表达和基因淘汰的功能分析.
- 定量实时PCR (qRT-PCR) 用于研究基因表达网络.
- 对胆汁表型和线虫繁殖的分析.
主要成果:
- 宫外ERF115表达导致过早的胆汁诱导,而PAT1上调诱导胆汁形成.
- ERF115和PAT1的淘汰突变体表现出较小的胆汁和减少的线虫繁殖.
- 似乎ERF115网络是胆汁中介的伤口感应和组织再生能力.
- 在胆汁中检测到CYCD6;1的高表达,而WIND1的过度表达模仿了ERF115过度表达的表现型.
结论:
- ERF115-PAT1复合体在协调植物应激反应与RKN胆汁形成期间的组织再生中发挥着关键作用.
- 这种复合物很可能促进胆汁的发育,确保线虫的生存和繁殖.
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