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相关概念视频

Replication in Prokaryotes02:35

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Overview
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Chromosome Structure02:40

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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
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The Replisome03:01

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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复制bSTAR序列和开源实现的复制.

Nam G Lee1,2, Grzegorz Bauman3,4, Oliver Bieri3,4

  • 1Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, California, USA.

Magnetic resonance in medicine
|December 4, 2023
PubMed
概括
此摘要是机器生成的。

研究人员成功地复制了使用开源工具进行胸部成像的平衡SSFP半辐射双回声成像技术 (bSTAR). 这种开源的bSTAR显示出与原始方法相比的图像质量和性能,验证了其可重现性.

关键词:
肺部的MRI 肺部的MRI 肺部的MRI这是一个开源的开源软件.复制复制复制复制复制复制复制可复制的研究研究.

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科学领域:

  • 医疗成像医学成像
  • 科学领域的开源软件
  • 磁共振成像是一种磁共振成像技术.

背景情况:

  • 科学可重复性对于知识的进步至关重要.
  • 这就是ISMRM.
  • 和我一起重复一下.
  • 挑战旨在评估方法复制.
  • 平衡的SSFP半辐射双回声成像技术 (bSTAR) 是胸部成像的一种新方法.

研究的目的:

  • 使用开源框架复制bSTAR成像技术.
  • 评估bSTAR在0.55T的胸部成像中的可重现性.
  • 将开源bSTAR实现的性能与原始方法进行比较.

主要方法:

  • bSTAR脉冲序列是使用开源Pulseq框架实现的.
  • 图像重建是使用开源的伯克利高级重建工具箱 (BART) 进行的.
  • 复制的开源bSTAR在健康志愿者身上进行了测试,并通过复制已发表的数据与原始bSTAR进行了比较.

主要成果:

  • 开源的bSTAR成功生成了没有可见文物的胸部图像.
  • 图像质量,空间分辨率和肺血管的明显性与原来的bSTAR相似.
  • 开源实现显示了足够的信号噪声比 (SNR) 和文物水平.

结论:

  • 使用开源框架 (Pulseq和BART) 成功复制了0.55T肺部成像的bSTAR技术.
  • 这项研究表明,复制出版物中描述的研究方法的可行性.
  • 这一成功为bSTAR成像技术的可复制性提供了信心.