通过SET7/9对表观遗传JMJD2D蛋白的甲基化促进前列腺瘤发生
Ruicai Gu1, Tae-Dong Kim1, Hanlin Jiang2
1Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States.
Frontiers in oncology
|December 4, 2023
概括
JMJD2D表观遗传调节器过度表达促进前列腺癌的生长. JMJD2D的氨酸甲基化影响癌细胞行为,并影响CBLC基因表达,突出显示前列腺癌治疗的潜在治疗标.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子瘤学分子瘤学
- 生物化学 生物化学
背景情况:
- 在前列腺癌中JMJD2D表观遗传调节者的作用尚不清楚.
- 了解JMJD2D调节对于确定新的治疗策略至关重要.
研究的目的:
- 研究前列腺癌中JMJD2D的调节和功能.
- 为了阐明JMJD2D活动对癌症进展的下游影响.
主要方法:
- 在前列腺瘤中分析JMJD2D表达.
- 在K427.2D的JMJD2D的局部定向突变发生.
- 细胞生长,入侵和瘤形成的测试.
- 转录基因分析.转录基因分析.
- 对基因表达的生物信息分析.
主要成果:
- 在前列腺瘤中,JMJD2D过度表达,促进癌细胞生长.
- 由SET7/9对JMJD2D在K427的氨酸甲基化调节了它的致癌功能.
- 在JMJD2D中K427突变会影响DU145细胞的生长,入侵和瘤形成.
- JMJD2D活动影响CBLC (一种泛素结合酶) 和PLAGL1 (一种转录因子) 的表达.
- 在前列腺瘤中,CBLC是上调的,其下调模仿了K427突变的影响.
结论:
- 通过氨酸甲基化确定了JMJD2D的新型调节机制.
- 在前列腺癌中JMJD2D的亲瘤作用部分通过调节CBLC表达来调节.
- 在前列腺癌中,CBLC表现出前列腺癌的前性作用,这表明它是潜在的治疗点.
- JMJD2D和CBLC抑制剂可能为前列腺癌治疗提供治疗效益.
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