相关实验视频

Updated: Jul 9, 2025

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

纳米颗粒 (NP) 介导的Siglec15沉默和巨细胞再极化,用于增强癌症免疫治疗

Xiaodi Liu^1,2, Qi Zhang^2,3, Yixia Liang³

¹Department of Ultrasound, Laboratory of Ultrasound Imaging and Drug, West China Hospital, Sichuan University, Chengdu 610041, China.

Acta pharmaceutica Sinica. B

|December 4, 2023

概括

向的纳米颗粒将干扰素- (IFNγ) 和siRNA传递给与瘤相关的巨细胞,增强T细胞透和增殖,以改善癌症免疫治疗结果,特别是在肝细胞癌中.

科学领域:

免疫学免疫学免疫学
纳米技术纳米技术
在瘤学瘤学.

背景情况:

对于癌症免疫疗法的成功,T细胞的透和增殖至关重要.
干扰素- (IFNγ) 促进T细胞的招募,而TAM中的Siglec15则抑制T细胞的增殖.
调节巨细胞功能提供了一种增强抗瘤免疫力的策略.

研究的目的:

开发用于IFNγ和Siglec15siRNA (siSiglec15) 的联合传递的降解反应纳米粒子.
通过同时促进T细胞透和增殖来增强癌症免疫疗法.
为了研究肝细胞癌 (HCC) 模型中的治疗潜力.

主要方法:

制造基于聚 (二硫化胺) 和脂质聚 (乙烯基醇) 的纳米粒子.
用siSiglec15和IFNγ.加载的纳米颗粒进行全身静脉注射.
评估纳米粒子积累,TAMs的细胞吸收,以及瘤组织中的载荷释放.
评估巨细胞再极化,T细胞反应和HCC瘤生长抑制.

主要成果:

纳米粒子有效地积聚在瘤组织中,并被TAMs内部化.
谷氨触发的IFNγ和siSiglec15再极化巨细胞的释放,增强T细胞的透和增殖.
联合治疗显著抑制了HCC瘤的生长,特别是免疫检查点抑制剂.

结论:

开发的纳米粒子有效地向TAM传递治疗有效载荷,以增强癌症免疫疗法.
该策略同时促进T细胞透和增殖,导致显著的瘤抑制.
这种方法代表了改善肝细胞癌治疗结果的有希望的工具.

关键词:

癌症免疫疗法癌症免疫疗法肝细胞癌是肝细胞癌.巨细胞再极化发生.纳米颗粒纳米颗粒在Siglec1515中使用.透T细胞的透情况在T细胞增殖过程中,T细胞的增殖与瘤相关的巨细胞.

更多相关视频

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...