肠道微生物群的变化与活跃结核病和潜伏结核病感染有关
Yuan Huang1,2, Jinhua Tang1, Zheng Cai1
1Department of Clinical Laboratory, Air Force Medical Center, Beijing, 100142, China.
Heliyon
|December 4, 2023
概括
结核病感染会改变肠道微生物群,减少多样性和特定的有益细菌. 某些肠道细菌显示出作为活性结核病 (ATB) 和潜在结核病感染 (LTBI) 的诊断标记物的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 结核病 (TB) 是由*Mycobacterium tuberculosis* (MTB) 引起的全球重大健康挑战.
- 肠道微生物群在调节宿主免疫系统和整体健康方面发挥着至关重要的作用.
- 在各种传染病中观察到肠道微生物群组成的变化,但它们在结核病原和诊断中的作用需要进一步阐明.
研究的目的:
- 与健康对照组 (HC) 相比,研究活跃结核病 (ATB) 和潜伏结核病感染 (LTBI) 患者的肠道微生物群的独特特征.
- 为了确定潜在的微生物生物标志物用于诊断ATB和LTBI.
- 探索ATB患者的肠道微生物群,宿主免疫反应 (T细胞) 和临床参数 (bilirubin) 之间的关系.
主要方法:
- 从19名ATB患者,21名LTBI患者和20名HC患者收集了便样本.
- 用16S rDNA测序分析了肠道微生物组成.
- 来自ATB患者的临床信息,包括免疫细胞计数和血清胆红素水平,被收集并与微生物数据相关联.
主要成果:
- 与HC相比,ATB和LTBI两组都表现出微生物多样性的减少和*Clostridia*的下降.
- ATB患者的蛋白质菌增加,而LTBI患者的*Prevotella*和*Rosburia*丰富.
- *Faecalibacterium*, *Clostridia* 和 *Gammaproteobacteria* 显示了ATB的诊断潜力 (AUC分别为0.808,0.784,0.717),以及LTBI的 *Prevotella* 和 *Rosburia* (两者的AUC为0.689).
结论:
- 在这项研究中发现的特定肠道细菌作为ATB和LTBI的有前途的诊断标记.
- 在MTB感染期间,肠道微生物群的组成发生了显著的变化.
- 某些肠道细菌可能通过与T细胞和胆红素代谢的相互作用影响抗MTB免疫力.
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