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在Mimulus guttatus中基因表达的定量遗传学

Paris Veltsos^1,2, John K Kelly¹

¹Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, KS, USA.

bioRxiv : the preprint server for biology

|December 4, 2023

概括

大多数基因表现出 cis 调节变异, cis 位置解释了显著的遗传变异. 转变作用的基因位点有助于基因表达相关性,可能塑造基因表达模块.

科学领域:

基因组学就是基因组学.
人口遗传学人口遗传学
分子生物学分子生物学

背景情况:

基因表达是由遗传变异调节的.
交叉作用的变体靠近基因,而跨作用的变体远离基因.
了解调节变异是解释特征遗传结构的关键.

研究的目的:

调查cis和trans调控变异对Mimulus guttatus基因表达的贡献.
描述cis和trans eQTLs (表达量化特征位点) 的性质和程度.
探索跨eQTLs在基因表达相关性和模块中的作用.

主要方法:

从自然的 Mimulus guttatus 种群创建了一个多父母的映射种群.
在1588种植物的叶子中得分基因表达.
绘制了 cis 和 trans eQTL 的地图,并分析了它们的特性.

主要成果:

几乎所有的测量基因 (91%) 都表现出 cis 调控变异.
Cis eQTL通常是具有多个功能性等位基因的等位基因序列.
Cis loci解释了约2/3的表达中的基因变异.
大多数基因变异来自转基因作用位仍然无法解释.
跨eQTL显示较低的等位基因多样性和更高的遗传优势比cis eQTLs.
跨eQTLs对表达中的遗传相关性有很大贡献.

结论:

基因调节变异是普遍存在的,也是基因表达中的常态遗传变异的主要来源.
很可能存在大量具有微妙影响的转基因作用基因储备.
转基因eQTLs在塑造基因表达模块及其演变方面发挥着重要作用.

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