氨酸的可用性会影响细胞分化过程中的基本H3K36me3动态
Yudong Sun1, Vijyendra Ramesh2, Fangchao Wei3
1Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina, USA.
bioRxiv : the preprint server for biology
|December 4, 2023
概括
氨酸的可用性动态调节H3K36me3,这是一个关键的表观遗传标记. 这一发现揭示了氨酸代谢如何通过染色质修饰影响细胞命运和肌性分化.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 细胞的新陈代谢
背景情况:
- 基因组蛋白修饰调节基因表达和细胞状态.
- 氨酸代谢会影响基因素甲基化,但其在特定基因素标记中的直接作用尚不清楚.
研究的目的:
- 为了研究氨酸可用性对H3K36me3的直接影响,这是一个关键的组蛋白标记物.
- 阐明 metionin代谢在肌体分化和细胞命运决定中的作用.
主要方法:
- 研究了 H3K36me3 通过甲和其代谢物 S-adenosylmethionine (SAM) 的动态调节.
- 研究了氨酸甲基转移酶SETD2在依赖 metionin 的分化中的作用.
- 分析了 metionin 限制对 H3K36me3 丰度和基因组可访问性的影响.
- 研究了Setd2删除对分化和染色质可访问性的影响.
主要成果:
- 甲氨酸动态调节H3K36me3,一种与癌症相关的组织蛋白修饰.
- 甲氨酸依赖的分化部分由SETD2.2介导.
- 氨酸的限制降低了H3K36me3和肌原基因中的染色质可访问性.
- Setd2删除副本 氨酸限制对分化和染色质可访问性的影响.
结论:
- 氨酸代谢直接影响细胞命运的决定,由染色质修饰酶感知.
- H3K36me3和SETD2是 metionin对肌体分化影响的关键媒介.
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