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Updated: Jul 9, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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在Steinernema中进行CRISPR-Cas9基因组编辑,该基因组编辑是针对Steinernema的虫病原性线虫

Mengyi Cao

    bioRxiv : the preprint server for biology
    |December 4, 2023
    PubMed
    概括

    研究人员开发了CRISPR-Cas9基因组编辑器,用于虫病原性线虫 (EPN). 这一突破使得研究这些重要的农业害虫的基因功能成为可能,为加强害虫控制策略铺平了道路.

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    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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    CRISPR01:59

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    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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    Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
    The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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