安菲奥克斯 (Amphioxus Gli) 淘汰会破坏左右不对称的发展,但对神经模式的影响有限
Xin Huang1, Qiongqiong Ren1, Yiquan Wang1
1State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, 361005 China.
Marine life science & technology
|December 4, 2023
概括
安菲奥克萨斯有一个Gli基因,该基因可分为Glis和Glil的两种异型. 淘汰赛显示,GliL对于左右不对称的发展至关重要,而这两种异构体都在神经管模式中发挥作用.
科学领域:
- 发展生物学 发展生物学
- 进化生物学 进化生物学
- 遗传学 遗传学 是一个
背景情况:
- 刺 (Hh) 信号传递对发育至关重要,由 Gli 转录因子介导.
- 脊椎动物拥有多个Gli对应物,有助于复杂的Hh反应.
- 一个早期的带动物Amphioxus提供了关于Gli基因与单个Gli基因的前重复基因演变的见解.
研究的目的:
- 为了研究 amphioxus Gli 异型体 (GliS 和 GliL) 在发育中的功能作用.
- 要了解与脊椎动物相比,Gli功能在进化过程中的分歧.
- 阐明Gli异型体对Hh信号通路的特定贡献.
主要方法:
- 产生了两个amphioxus Gli淘汰突变:一个全基因淘汰和一个GliL特定的淘汰.
- 对淘汰胚胎的形态和分子缺陷的分析.
- 对已知Hh通路突变的表型进行比较分析.
主要成果:
- 两次Gli淘汰赛都在左右不对称发展方面表现出显著的缺陷.
- 单独的 GliL 淘汰就不会影响神经管模式,与全 Gli 淘汰不同.
- 神经管模式显示,在全Gli淘汰赛中出现了轻微的变化,影响了Olig基因表达.
结论:
- 两种Amphioxus Gli异型 (GliS和GliL) 具有不同的,部分冗余的功能.
- GliL在建立左右不对称方面发挥着关键作用.
- Gli 异构体的差异性功能有助于状动物进化中的 Hh 信号的复杂性.
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