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皮肤病毒的进化远端尾部蛋白质促进了对外聚糖编码乳球菌的吸附
Anne M Millen1, Damian Magill2, Dennis Romero1
1Health and Biosciences, IFF, Madison, WI 53716, USA.
Microbiome research reports
|December 4, 2023
概括
乳球菌皮肤病毒在乳制品环境中面临挑战. 一种特定的外聚糖 (EPS) 阻断了一些菌体,而另一些菌体进化了更长的尾巴蛋白来结合EPS,增强了感染.
科学领域:
- 微生物学和病毒学
- 菌体与宿主之间的相互作用
- 工业生物技术 工业生物技术
背景情况:
- 乳球菌皮肤病毒在工业乳制品环境中具有重要意义.
- 菌体-宿主识别依赖于菌体蛋白和宿主细胞表面结构之间的相互作用.
- 乳球球菌等离子体pEPS6073编码了一个细胞表面的外聚糖 (EPS),称为6073-like.
研究的目的:
- 为了研究类似于6073的EPS对skunavirus敏感性的影响.
- 了解菌体远端尾部蛋白 (Dits) 在克服EPS介导抗性的作用.
主要方法:
- 伴侣转移pEPS6073进入模型乳球菌株.
- 菌体斑块和吸附试验以确定菌体的敏感性.
- 对菌体远端尾部蛋白质 (Dits) 的生物信息分析 (HHpred,AlphaFold).
- 用进化的Dit蛋白构建重组菌体.
主要成果:
- 类似于6073的EPS通过抑制菌体吸附,使其对一组斯库纳病毒产生抗性.
- 感染EPS产生菌株的skunavirus具有具有碳水化合物结合域的进化Dits.
- 具有进化Dits的重组菌体显示出恢复感染性和改善对EPS产生宿主的吸附性.
结论:
- 与6073类似的EPS可能会物理地阻断具有经典Dits.的菌体的菌体受体.
- 感染EPS产生的菌株的皮肤病毒中的进化Dits可能会促进对EPS屏障的吸附.
- 这项研究阐明了乳球菌中的菌体-宿主动态,并突出了DIT蛋白进化的作用.
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