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Updated: Jul 9, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

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在PEG-Ficoll原细胞中核酸分离

Tasdiq Ahmed1, Yan Zhang2, Ji-Hoon Lee1

  • 1Wallace H Coulter Department of Biomedical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology and Emory School of Medicine, Atlanta, GA, USA.

Journal of chemical and engineering data
|December 4, 2023
PubMed
概括

生物添加剂增强了聚合物基原细胞的稳定性和功能. 这项研究详细介绍了盐和有机分子如何改善相分离和核酸分离,以便更好地进行原细胞工程.

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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