在抗生素治疗小鼠口服VSL#3®后的益生菌殖民动态
Casey Theriot1, Rajani Thanissery1, Sarah O'Flaherty2
1Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27695, USA.
Microbiome research reports
|December 4, 2023
概括
在VSL#3中的益生菌菌株殖民于肠道,有些菌株殖民于早期,而另一些菌株则殖民于晚些时候. 这种益生菌补充剂增加了肠道微生物的多样性和特定的有益细菌,可能增强了对诸如Clostridioides difficile等病原体的抵抗力.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 益生菌的有效性取决于宿主胃肠道内的生存和殖民.
- 了解特定益生菌菌株的殖民动态对于它们的健康益处至关重要.
- 商用益生菌VSL#3含有多种菌株,可能对肠道微生物群产生影响.
研究的目的:
- 为了研究VSL#3配方内的单个益生菌菌株的殖民模式.
- 评估VSL#3消费对宿主肠道微生物群组成的影响.
- 评估VSL#3在增强对肠道病原体的耐药性的潜力.
主要方法:
- 一个接受了塞福拉治疗的小鼠模型被用来模拟抗生素诱导的肠道失调症.
- 每天服用大约10^8 CFU的VSL#3的口服测量剂,持续7天.
- 使用基于16S的便样本的amplicon测序来分析肠道微生物组成.
主要成果:
- 来自VSL#3的单个益生菌菌株显示过渡殖民,有明显的早期 (例如,Streptococcus thermophilus,Lactiplantibacillus plantarum) 和晚期 (例如,Lacticaseibacillus paracasei,Bifidobacterium breve) 殖民者被确定.
- 消费VSL#3导致内微生物群体的α多样性增加,特别是在抗生素治疗期间.
- 益生菌补充促进了蛋白质菌,细菌菌和动菌菌的扩张,包括Bifidobacteriaceae和Lachnospiraceae,这些与Clostridioides difficile耐药性有关.
结论:
- 该研究强调了评估益生菌菌株殖民能力及其对肠道微生物群的影响的重要性.
- 对于持续的短暂殖民和潜在的健康益处,可能需要多剂量VSL#3.
- 需要进一步的研究来证实VSL#3在预防Clostridioides difficile殖民和相关疾病的有效性.
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