Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
RNA Structure01:19

RNA Structure

4.8K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.8K
RNA Stability01:53

RNA Stability

33.6K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.6K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The RNA-Binding Protein PARN Remodeled 3' UTR Structure Defines Poly(A)-Loading Sites to Mediate Immunoglobulin Homeostasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

OSK-mediated partial reprogramming induces cardiomyocyte dedifferentiation, overcomes cytokinesis barriers, and promotes post-MI endogenous cardiac regeneration.

Journal of molecular and cellular cardiology·2026
Same author

m<sup>6</sup>A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo development.

The EMBO journal·2026
Same author

The effects of drinking water with essential oils-organic acids blend replacing preventive antibiotics during withdrawal period on growth performance and gut health of broilers in a commercial farm.

Poultry science·2026
Same author

Ancestral intronic splicing regulatory elements in the SCNα gene family.

RNA (New York, N.Y.)·2026
Same author

ssG4-seq for global profiling of strand-specific G-quadruplex structures in mammalian genomes.

Nature communications·2025

相关实验视频

Updated: Jul 9, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K

人类转录组中的远程RNA结构超出了进化保护区域.

Sergey Margasyuk1, Lev Zavileyskiy1, Changchang Cao2

  • 1Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Moscow, Russia.

PeerJ
|December 4, 2023
PubMed
概括

本研究介绍了PHRIC,这是一种计算工具,可以使用RIC-seq实验中的RNA接触来预测超出保护区域的RNA结构. PHRIC确定了成千上万的稳定,远程RNA结构,包括那些内部的内.

关键词:
另一个替代拼接.长距离的远程飞机亲近结合方式的结合.在RIC-seqq.结构 RNA 结构 RNA 结构有关RNA的接触者.

更多相关视频

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

289
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.7K

相关实验视频

Last Updated: Jul 9, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K
Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
07:28

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library

Published on: January 10, 2025

289
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

23.7K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 计算生物学 计算生物学

背景情况:

  • RNA结构对于基因调节至关重要,但由于大,不连续的基因,很难在真核生物中预测.
  • 现有的方法,如热力学建模和比较基因组学在预测RNA结构在不同的基因组区域有局限性.

研究的目的:

  • 开发一个计算管道 (PHRIC) 来预测远程RNA结构,超出保存的基因组区域.
  • 为了利用RNA在位构成测序 (RIC-seq) 的RNA接触来进行结构预测.
  • 分析人类细胞系中预测的RNA结构的流行率和特征.

主要方法:

  • 开发了PHRIC计算管道,以提取RNA片段,并根据RIC-seq.的RNA接触数据预测互补的基配对.
  • 在7个人类细胞系的RIC-seq实验中应用PHRIC.
  • 分析了稳定性 (自由能量<-15kcal/mol) 和保存模式的预测结构.

主要成果:

  • PHRIC预测大约有12,000个稳定的远程RNA结构,其中大多数位于保护的脊椎动物区域之外.
  • 预测的结构在其他类别中表现出序列保护和补偿替代.
  • 内子表现出更高的倾向形成稳定的远程RNA结构,通常在同一内子内.

结论:

  • PHRIC成功地将RNA结构预测扩展到保护区域之外,克服了以前方法的局限性.
  • 这些发现揭示了非保存区域中大量的功能性RNA结构,特别是在内部.
  • 这项工作证明了近距离结合试验对于全面的RNA结构发现的有用性.