Lysinoalanine 交叉链接是螺旋体鞭毛的保存后翻译性修饰
Michael J Lynch1, Maithili Deshpande1, Kurni Kurniyati2
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
Lysinoalanine (Lal) 交叉链接对于各种物种的螺旋体运动是必不可少的. 在鞭毛蛋白中这种保存的修饰为新型抗微生物疗法提供了潜在的点,用于对抗莱姆病和白螺杆菌病等疾病.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 螺旋虫是一种致病性细菌,对莱姆病,螺杆菌病和梅毒等疾病负责.
- 与其他细菌不同,螺旋细胞鞭毛位于周等离子体空间内,通过独特的机制使其能够运动.
- 之前的研究已经确定了Treponema denticola和Borreliella burgdorferi的鞭毛蛋白 (FlgE) 中的共价氨酸氨酸 (Lal) 交叉链接.
研究的目的:
- 在各种螺旋体物种中调查鞭毛蛋白 (FlgE) 中氨酸 (Lal) 交叉链的存在和必要性.
- 为了确定Lal交叉链接是否是Spirochete类中的保存后翻译修饰.
- 评估Lal交叉链的潜力,作为开发螺旋体特异性抗菌剂的目标.
主要方法:
- 从多种螺旋体物种 (*Treponema*, *Borreliella*, *Brachyspira*, *Leptospira*) 的复合和体内衍生样本进行分析.
- 质谱测量用于识别和表征FlGE中的lysinoalanine (Lal) 交联.
- 使用野生类型和突变型螺旋体菌株进行移动性测定,这些菌株缺乏形成Lal交叉链路的能力.
主要成果:
- 在FlgE中证实了来自*Treponema* spp.,*Borreliella* spp.,*Brachyspira* spp.和*Leptospira* spp.的酸 (Lal) 交联酸.
- 一种无法形成Lal交叉链的*Borreliella burgdorferi*突变体表现出明显的运动能力受损.
- 虽然 *Leptospira* spp. 在 由于flgE缺乏Lal形成的保存的氨酸残留物,检测到了替代的Lal异型,表明了特定物种的变异.
结论:
- lysinoalanine (Lal) 交叉链是维护和必要的翻译后修改,用于横功能和运动性.
- 在各种螺旋体中发现的Lal交叉链的存在表明,它们对鞭毛的结构完整性和功能至关重要.
- 拉尔交叉链路是开发新型,螺旋体特异性抗菌剂的有希望和保守的目标.
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