内生ASC斑点的纳米级组织
Ivo M Glück1,2, Grusha Primal Mathias1, Sebastian Strauss3,4
1Department of Chemistry, Ludwig Maximilians-Universität München, Butenandtstraße 5-13, 81377 München, Germany.
iScience
|December 4, 2023
概括
在NLRP3炎症酶.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
背景情况:
- NLRP3炎症酶对天生的免疫非常重要.
- ASC斑点的形成是炎症酶激活的关键.
- 以前的ASC点模型依赖于过度表达.
研究的目的:
- 使用内生ASC可视化ASC斑点结构.
- 为了阐明ASC斑的超分子组织.
主要方法:
- 使用双色 3D 超高分辨率显微镜 (dSTORM 和 DNA-PAINT).
- 在本地可视化中使用内生ASC表达式.
- 用抗体和纳米体进行应用双重标记,以获得完整的结构捕获.
主要成果:
- 可视化的ASC斑点直径在800~1000nm之间.
- 在ASC斑点结构中观察到一个密集的核心,出现了细丝.
- 确定的ASC斑点在形成过程中变得更密,而不是更大.
- 在以后的时间点中没有发现与微管组织中心的协同定位.
结论:
- 提供了内生ASC斑块的高分辨率结构.
- 揭示了ASC斑点组装的动态性质.
- 提供了关于炎症组合的复杂形成和先天免疫信号的见解.
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