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相关概念视频

CRISPR01:59

CRISPR

51.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Homologous Recombination02:31

Homologous Recombination

50.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.6K

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相关实验视频

Updated: Jul 9, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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piCRISPR:基于物理信息的深度学习模型用于CRISPR/Cas9的目标外切割预测.

Florian Störtz1, Jeffrey K Mak1, Peter Minary1

  • 1Department of Computer Science, University of Oxford, Parks Road, Oxford OX1 3QD, UK.

Artificial intelligence in the life sciences
|December 4, 2023
PubMed
概括

这项研究介绍了piCRISPR,这是一个新的深度学习模型,用于预测CRISPR/Cas基因编辑的目标外影响. 它利用物理信息特征来提高预测准确度,这对于安全的体内基因疗法至关重要.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?这就是Cas9的情况.裂纹预测的预测深度学习是一种深度学习.核组织组织

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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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相关实验视频

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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因编辑技术的技术

背景情况:

  • 克里斯普尔/卡斯系统是具有治疗潜力的强大基因编辑工具.
  • 非目标编辑在体内基因编辑应用中构成重大安全问题.
  • 现有的预测算法通常不充分利用物理信息的特征.

研究的目的:

  • 开发一个先进的目标外预测模型,结合物理信息的特征.
  • 为了提高预测CRISPR/Cas核酶分裂活动的准确性.
  • 提高基因编辑用于治疗应用的安全性和有效性.

主要方法:

  • 实施最先进的深度学习算法.
  • 利用物理信息的特征,捕捉分离地点的生物环境.
  • 关于全面的crisprSQL目标外断裂数据集的培训和评估.

主要成果:

  • piCRISPR模型在目标外预测方面表现出很高的性能.
  • 物理信息的特征,包括序列上下文和染色质可访问性,对于准确的预测至关重要.
  • 新的功能显著提高了序列相同的位置对的预测准确性.

结论:

  • piCRISPR提供了一种更准确的方法来预测CRISPR/Cas的脱效应.
  • 该模型的环境敏感特征对于临床指南设计至关重要.
  • 这种方法提升了体内基因编辑疗法的安全性.