不仅仅是纤维性:内皮衍生TGFβ在恒常状态期间保持收缩功能和淋巴肌的表型
Miriam Carrillo Diaz de Leon1, Keith Keane2, Simon Roizes2
1Inflammation Research Network, Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases, Department of Microbiology, Immunology & Infectious Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
American journal of physiology. Cell physiology
|December 4, 2023
概括
转化生长因子-β (TGFβ) 对于淋巴血管功能至关重要,维持肌肉细胞收缩性和血管送. 这项研究揭示了淋巴内皮细胞是内源性TGFβ的关键来源,对淋巴平衡至关重要.
科学领域:
- 血管生物学 血管生物学
- 细胞信号传递 细胞信号传递
- 淋巴系统研究 淋巴系统研究
背景情况:
- 在恒常状态期间,在淋巴血管中细胞与细胞的通信尚未完全理解.
- 转化生长因子β (TGFβ) 主要以其在血管炎症和纤维化中的病理作用而闻名,对其在淋巴血管中的平静功能的洞察力有限.
研究的目的:
- 研究TGFβ信号在淋巴血管系统的恒温功能中的作用.
- 为了确定淋巴血管内内源性TGFβ的来源和调节.
- 阐明TGFβ对淋巴肌细胞表型和血管收缩性的影响.
主要方法:
- 在老鼠中腔淋巴血管中对TGFβ受体1 (TGFβR1) 的药理阻塞.
- 在体外分析初级分离的淋巴肌细胞.
- 介质淋巴细胞内皮细胞TGFβ产生的评估.
- 研究氧化在调节TGFβ产生中的作用.
主要成果:
- 阻断TGFβR1显著降低了淋巴血管收缩性和抽血,损害了肌肉覆盖.
- 介质淋巴细胞内皮细胞被确定为内源性TGFβ的来源.
- 淋巴内皮细胞的TGFβ产生在模式识别受体激活后增加.
- 持续的TGFβ供应对于维持淋巴肌细胞收缩表型至关重要,在体外观察到的救援.
- 发现氧化可以调节淋巴内皮TGFβ的产生.
结论:
- 恒常性TGFβ信号传递对于维持淋巴血管功能,特别是收缩性和送,至关重要.
- 调节失调或抑制TGFβ信号传递可能会对淋巴血管健康产生负面影响.
- 淋巴内皮细胞在产生和调节TGFβ中起着至关重要的作用,以支持淋巴肌功能.
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