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牛卵细胞中的细胞质颗粒不会影响胚胎或胎儿的发育

Paola Maria da Silva Rosa^1,2, Pedro Henrique Evagelista Guedes¹, Joaquim Mansano Garcia²

¹Embrapa Dairy Cattle, 610 Eugenio do Nascimento Ave, Juiz de Fora, MG, Brazil36038-330.

Zygote (Cambridge, England)

|December 4, 2023

概括

具有颗粒细胞质的牛卵细胞与具有均质细胞质的卵细胞相比,显示出类似的发育能力和妊娠结果. 尽管有分子差异,但颗粒状卵子细胞支持高效的体外受精和小牛生产.

科学领域:

生殖生物学生殖生物学
动物科学动物科学
卵细胞生物学卵细胞生物学

背景情况:

卵细胞的细胞质外观,特别是同质性和颗粒性,是评估卵细胞能力的关键因素.
不同质的颗粒状细胞质对牛卵细胞质量和发育潜力的影响仍然不完全理解.

研究的目的:

研究粒状细胞质对牛卵细胞能力和发育潜力的影响.
为了比较具有均质和颗粒状细胞质的卵子细胞之间的分子标记物和体外/体内发育速率.

主要方法:

从两个来源:屠宰场卵巢和吉罗兰多卵子采集中选择具有同质 (控制,CC) 和颗粒 (GC) 细胞质的牛积卵细胞复合体 (COC).
对酶3水平,GAP结活动和特定基因的相对mRNA表达 (ZAR1,MATER,PPP2R1A,ENY2,IGF2R,BMP15) 的分析.
评估体外受精 (IVF) 参数,包括切割和胚胎细胞分裂率,以及体内胚胎发育,包括移植后的怀孕和分娩率.

主要成果:

与对照卵子细胞相比,颗粒卵子体表现出较高的caspase 3水平,但与对照卵子体相比,具有类似的GAP结活动.
在颗粒卵细胞中观察到较低的ZAR1mRNA水平,而在其他测试基因中没有显著差异.
卵细胞的裂变,胚胎细胞率,怀孕率,分娩率和胎儿生长在具有均和颗粒细胞质的卵细胞之间是可比的.

结论:

具有颗粒细胞质的卵子体表现出相当于牛同质细胞质的卵子体的发育能力和生殖效率.
尽管观察到分子差异,但细胞质颗粒度不会对卵细胞质量,试管婴儿成功或小牛生产产生不利影响.
这些发现表明,细胞质颗粒度不是限制卵细胞在牛繁殖中的发育能力的限制因素.

关键词:

颗粒颗粒是什么颗粒颗粒在这个过程中,IVF IVF.形态分类形态分类形态分类卵细胞细胞质细胞质.蛋细胞质量质量质卵子质卵子是什么

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