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相关概念视频

Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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基于微板的细胞活力检测作为微藻分析流细胞计的经济有效替代方案.

Diyuan Wang1, Francis L de Los Reyes1, Joel J Ducoste1

  • 1Department of Civil, Construction, and Environmental Engineering, North Carolina State University, Raleigh, North Carolina 27606, United States.

Environmental science & technology
|December 4, 2023
PubMed
概括

新的微板测定提供了一种低成本,简单的方法来评估微藻细胞活力,这对于生物炼油和生物修复应用至关重要. 这种技术提供了可靠的结果,与昂贵的流细胞计量相提并论.

关键词:
杜纳利拉病毒 (Dunaliella viridis) 是一种细胞活力的细胞活力.红色素 B B 红色素 B光素二甲酸盐 (FDA) 的使用.光染料是一种光染料.微藻是一种微藻.微板阅读器 微板阅读器

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科学领域:

  • 生物技术是生物技术.
  • 微藻养殖 微藻养殖
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞活力对于生物炼油厂和生物修复中的微藻种植至关重要.
  • 目前用于评估可行性的流细胞计方法是昂贵的,并且不适合工业用途.
  • 需要开发具有成本效益和可访问的可行性分析.

研究的目的:

  • 开发一种具有成本效益和简单的微板测试,以检测微藻细胞活性的方法.
  • 为了验证测试对流细胞计的性能.
  • 提供关于微藻染色协议的指导.

主要方法:

  • 开发了使用光素二甲酸盐 (活色) 和红素B (死色) 的微板试验.
  • 应用测试来监测Dunaliella viridis*在营养压力下的生存能力.
  • 系统地研究着染料度,化时间和背景光效应.

主要成果:

  • 微板测量精确量化的细胞活力,显示与流细胞计的良好一致.
  • 在碳和限制下监测微藻的成功应用.
  • 确定了测试性能的最佳条件.

结论:

  • 开发的微板测试适用于微藻种植的工业应用.
  • 这种方法为未来的微藻生存能力评估和应用提供了宝贵的见解.
  • 为生物炼油厂和生物修复工艺的质量控制提供了一个实用的工具.