从水溶性块共聚物中释放光刺激的扩展氧化 (NO),以增强抗菌活性
Soumya Paul, Priya Ashrit1, Manish Kumar
1Institute for Stem Cell Science and Regenerative Medicine (InStem), UAS-GKVK Campus, Bellary Road, Bangalore 560065, Karnataka, India.
Biomacromolecules
|December 4, 2023
概括
两块共聚合物在暴露于光线时可控地释放氧化 (NO). 这种持续的NO释放显示出对 Staphylococcus aureus 的抗菌活性增强.
科学领域:
- 聚合物化学 聚合物化学
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 已知N-Nitrosamines是具有光标记性的NO释放的基因.
- 控制的氧化 (NO) 输送对于各种生物医学应用至关重要.
- 开发具有可调节释放动力学的光触发NO捐赠体是一个活跃的研究领域.
研究的目的:
- 为了合成和描述基于N-胺胺的双胞胎块共聚合物 (BCP) 用于受控的光触发NO释放.
- 为了研究来自BCP细胞的NO释放机制和动力学.
- 评估NO释放BCP与小分子NO供体 (NOD) 相比的抗菌疗效.
主要方法:
- 基于N-尼托胺的两性阻断共聚合物 (BCP) 的合成.
- 在水性介质中的BCP状结构的表征.
- 使用UV-Vis光谱学在365nm光辐射下释放的氧化 (NO) 的量化.
- 电子磁共振 (EPR) 光谱检测,以确认释放的NO的激进性质.
- 在体外抗菌测定对金黄色葡萄球菌.
主要成果:
- 合成的BCP在水中形成稳定的菌根结构.
- 在光照照射后,BCP表现出持续的NO释放 (92-160μM超过11.5小时,理论产量的36.8-64.0%).
- 一个小分子NO供体 (NOD) 在2.5小时内在DMSO中显示出快速的NO释放.
- 释放的NO的激进性质得到了EPR的证实.
- 与NOD相比,从BCP中持续释放的NO显示出对黄金葡萄球菌的优越抗菌活性.
结论:
- 基于N-氨酸胺的双胞胎区块共聚合物提供了一个可控的,光触发的氧化 (NO) 输送平台.
- BCP的状结构促进了持续的NO释放,增强了抗菌功效.
- 这项研究强调了可调节的NO释放聚合物的潜力,用于开发先进的抗微生物策略.
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